NUÔI CẤY HUYỀN PHÙ VÀ TẠO KEO TỤ LẮNG TẢO CHLORELLA VULGARIS CHO NUÔI CẤY CỐ ĐỊNH TRONG BIOFILM PHOTOBIOREACTOR PHƯƠNG NGHIÊNG
Tóm tắt
Công nghệ nuôi vi tảo Chlorella vulgaris cho thấy có nhiều tiềm năng trong việc xử lí nước thải song song với thu sinh khối để sản xuất nhiên liệu sinh học. Việc nuôi tảo kiểu huyền phù, tự dưỡng ở nhiều quy mô khác nhau vẫn là phương thức chủ yếu để tăng sinh khối trong các giai đoạn của công nghệ nuôi tảo. Trong nghiên cứu này, tảo C. vulgaris được nuôi tăng sinh trong hệ thống huyền phù 2 l để chuẩn bị sinh khối cho nuôi tảo kiểu cố định trong hệ thống biofilm photobioreactor phương nghiêng. Mật độ tế bào ban đầu và cường độ ánh sáng thích hợp cho nuôi huyền phù được xác định là 5.105 tế bào/ml và 120 µmol photon/m2/s. Sau 20 ngày nuôi, mật độ sinh khối khô vi tảo trong hệ thống 2 l đạt 0,315 g/l. Để cô đặc huyền phù tảo chuẩn bị sinh khối cho cố định thành biofilm, phèn chua (KAl(SO4)2) được sử dụng ở các nồng độ từ 0,2 đến 2 g/l để tạo keo tụ lắng. Nồng độ phèn chua 0,2 g/l cho thấy hiệu quả thu tảo đến 95% và tảo thu được có khả năng tăng sinh mạnh khi được chuyển nuôi cố định trong biofilm photobioreactor phương nghiêng.
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DOI: https://doi.org/10.54607/hcmue.js.19.3.3170(2022)
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