PHÂN LẬP VÀ TUYỂN CHỌN MỘT SỐ DÒNG VI SINH VẬT PHÂN GIẢI CHẤT HỮU CƠ TỪ BÙN THẢI AO NUÔI TÔM Ở HUYỆN CẦN GIỜ
Tóm tắt
Hệ vi sinh vât trong bùn thải ao tôm có khả năng phân giải các chất hữu cơ gây ô nhiễm môi trường nuôi. Nhóm vi sinh vật này là nguồn nguyên liệu hữu ích để sử dụng sản xuất chế phẩm xử lí ô nhiễm môi trường nuôi trồng thuỷ sản. Đề tài thu thập 16 mẫu bùn thải ao nuôi tôm ở huyện Cần Giờ, Thành phố Hồ Chí Minh và tuyển chọn được 51 chủng vi khuẩn, 9 chủng nấm men và 19 chủng xạ khuẩn có khả năng chịu mặn ở 30‰. Hai chủng vi khuẩn B25, B29 và 3 chủng xạ khuẩn X17, S11 , S1 có hoạt tính protease tốt nhất. 2 chủng xạ khuẩn S1, S11 và chủng nấm men N1 là có hoạt tính cellulase tốt nhất. 2 chủng vi khuẩn B29, B28 và chủng xạ khuẩn S11 có hoạt tính amylase tốt nhất. Bộ sưu tập vi sinh vật của đề tài có khả năng sử dụng trong nghiên cứu sản xuất chế phẩm sinh học, cải tạo môi trường nuôi trồng thuỷ sản
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Ashwini, K., & Sampathkumar, S. (2014). Isolation and screening of potent amylase-producing marine actinomycetes from seabed. Malaya Journal of Biosciences, 1(4), 273-276.
Das, P., Solanki, R., & Monisha, K. (2014). Isolation and screening of cellulolytic actinomycetes from diverse habitats. International Journal of Advanced Biotechnology and Research, 5(3), 438-451.
Gebreyohannes, G. (2015). Isolation and optimization of amylase producing bacteria and actinomyces from soil samples of Maraki and Tewedros campus, University of Gonda, north west Ethiopia. African Journal of Microbiology Research, 9(31), 1877-1882
George, M. G. (2005). Bergey's manual of systematic bacteriology. United States.
Gothandam, K. M., Suganthi, C., Mageswari, A., Karthikeyan, S., Anbalagan, M., & Sivakumar, A. (2013). Screening and optimization of protease production from a halotolerant Bacillus licheniformis isolated from saltern sediments. Journal of Genetic Engineering and Biotechnology, 11(1), 47-52.
Holmberg, S. D. (1988). Vibrios and Aeromonas. Infect Dis Clin North Am, 2(3), 655-76.
James, R. F., Byron, S. M., & John, A. Z. (1961). A method for the determination of relative amounts of malted-wheat, fungal (Aspergillus oryzea) and bacteria (Bacillus subtilis) alpha-amylase in mixcultures, and its application to malted wheat. Cereal Chemistry, 38, 479-486.
Jayasree, D., Sandhya Kumari, T. D., Kavi Kishor, P. B., Vijaya Lakshmi, M., & Lakshmi Narasu, M. (2009). Optimization of production protocol of alkaline protease by Streptomyces pulvereceus. InterJRI Science and Technology, 1(2), 79-82.
Khuat, H. T., & Le, A. X. (2012). Isolation and selection of Bacillus strains for the usage in sludge treatment in intensive shrimp ponds p. Science and Technology Journal of Agriculture & Rural Development, 2, 86-90.
Lodder, J. (1970). The yeast, A taxonomic study. North-Holland, Amsterdam.
Mohanta, Y. K. (2014). Isolation of cellulose-degrading actinomycetes and evaluation of their cellulolytic potential. Bioengineering and Bioscience, 2(1), 1-5
Narendhirakannan, R. T., Sudarshan, S. R., & Mannivannan, A. (2014). Screening of cellulase producing microorganisms from lake area containing water hyacinth for enzymatic hydrolysis of cellulose. Journal of Advanced Scientific Research, 5(3), 23-30.
Nguyen, D. L., Phan, T. H., & Nguyen, A. T. (2011). Thi nghiem cong nghe sinh hoc. Tap 2. Thi nghiem vi sinh vat hoc [Biology listening test. Volume 2. Microbiological testing]. Ho Chi Minh City National University Press.
Nguyen, P. B. (2011). Nghien cuu de xuat cac giai phap tong hop xu ly bun ao nuoi tom o huyen Can Gio [Research and propose integrated solutions for shrimp pond sludge treatment in Can Gio district]. Acceptance report, Institute of Tropical Technology and Environmental Protection, Ho Chi Minh City.
Nguyen, T. Q. T. (2011). Tuyen chon cac chung vi sinh vat tao che pham nham xu li nuoc thai nuoi trong thuy san [Selection of microbial strains to create products to treat aquaculture wastewater]. Master thesis. VNU University of Science, Hanoi National University.
Nguyen, V. P., & Phan, T. P. T. (2014). Phan lap, dinh danh va xac dinh các dac tinh co loi của chủng Bacillus spp. tu ao nuoi tom o tinh Ben Tre [Isolating, identifying and determining the beneficial properties of Bacillus spp. strains from shrimp ponds in Ben Tre Province]. Ho Chi Minh City University of Education Journal of Science, 64, 94-102.
Nguyen, T. D. H., Hua, T. C., Dang, B. N., Nguyen, T. T. T., Nguyen, N. A., & Pham, T. V. (2021), Isolation and selection of actinomycetes producing high bioactive substances. Journal of Science and Technology, 53B, 56-67.
Pham, T. T. N., & Truong, Q. P. (2010). Bien dong cac yeu to moi truong trong ao nuoi tom su (Penaeus monodon) tham canh tai Soc Trang [Study on water quality of intensive shrimp (Penaeus monodon) ponds in Soc Trang province]. Journal of Science, Can Tho University, 15a, 179-188.
Pham, T. N. L., Nguyen, H. H., & Ngo, T. T. C. (2013). Khao sat buoc dau vi sinh vat phan giai tinh bot o mot so ao nuoi tom thuoc dam Sam-Chuon, Phu Vang, Thua Thien – Hue [Preliminary investigation on starch degrading microorganisms in shrimp ponds at Sam-Chuon lagoon, Phu Vang, Thua Thien – Hue]. In The 5th national scientific conference on ecology and biological resources (pp. 1116-1121).
Phan, T. H. N., & Pham, K. L. (2012). Study of wastewater treatments from brackish aquaculture using a submered arerated fixed bed reactor. College of Sciences, Hue University, 74B(5),
-122.
Prabavathi, R., Mathivanan, V., & Ambika, A. (2012). Screening of protease enzyme by construction of metagenomic library from marine soil sediments. International Journal of Pharma Sciences and Research, 3(7), 396-399.
Shanmugapriya, K., Saravana, P. S., Krishnapriya, Manoharan, M., Mythili, A., & Joseph, S. (2012). Isolation, screening and partial purification ofcellulase from cellulase producing bacteria. International Journal of Advanced Biotechnology and Research, 3(1), 509-514.
Sumithra, V., Sudha, S. S., & Jayasankar, N. S. (2015). Isolation, screening and assay of halophilic protease from saltpan samples of tuticorin district. Scrutiny International Research Journal of Microbiology and Biotechnology, 2(2), 7-12.
Tansel, Y. H. (2013). Isolation and characterization of amylase producing yeasts and improvement of amylase production. Turkish Journal of Biochemistry, 38(1), 101-108.
Viswanathan, K., Jeyanthi Rebecca, L., Arumugam, P., & Anbarasu, K. (2015). Isolation and screening of protease producing marine actinomycetes from Chennai coastal region. International Journal of Advanced Research in Biological Sciences, 2(8), 153-157.
Vo, H. T., Nguyen, H. M., & Nguyen, P. H. (2012). Hoat tinh protease cua mot so chung Bacillus phan lap tu nuoc thai che bien thit va thuy hai san [Study of protease activity of several Bacillus strains isolated from slaughterhouse and seafood processing wastewater]. VNU Journal of Science, 28, 116-124
Vu, T. L. P. (2015). Selection of lactic bacteria with high organic matter degrading activity from shrimp pond sludge in Thua Thien – Hue, Student scientific conference, Hanoi National University.
Waksman, S. A. (1961). The Actinomycetes. Vol. II. Classification, identification and descriptions of genera and species (ix+363 pp.). London: Baillière, Tindall & Cox, Ltd..
Wyban, J. A., Sweeney, J. N., & Kanna, R. A. (1988). Shrimp Yields and Economic Potential of Intensive Round Pond Systems. Journal of the World Aquaculture Society, 19(4), 210-217.
Zin, L. M. M., Win, M. T., & Myo, M. (2015). Study on the cellulase enzyme producing activity of bacteria isolated from manure waste and degrading soil. International Journal of Technical Research and Applications, 3(6), 165-169.
DOI: https://doi.org/10.54607/hcmue.js.20.11.3829(2023)
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