KHẢO SÁT ĐIỀU KIỆN NUÔI CẤY VÀ NGUỒN DINH DƯỠNG ẢNH HƯỞNG ĐẾN QUÁ TRÌNH SINH TỔNG HỢP ENZYME TIÊU SỢI HUYẾT CỦA CHỦNG Bacillus sp. ES4
Tóm tắt
Vi khuẩn Bacillus sp. có khả năng sinh tổng hợp enzym tiêu sợi huyết. Điều kiện nuôi cấy và nguồn dinh dưỡng là những yếu tố quan trọng ảnh hưởng đến sự phát triển và sản xuất enzyme của chủng. Vì vậy, trong nghiên cứu này, chúng tôi khảo sát điều kiện nuôi cấy của chủng Bacillus sp. ES4 như nhiệt độ, pH, nguồn dinh dưỡng C (glucose, sucrose, glycerol và maltose), nguồn dinh dưỡng N (cao nấm men, cao thịt, peptone và tryptone), các ion kim loại (Ca2+, Mg2+, Mn2+, Zn2+, K+, Fe2+ và Cu2+) và thời gian thu nhận sản phẩm enzyme. Kết quả cho thấy đối với Bacillus sp. ES4, nguồn C tốt nhất là glucose; nguồn N tốt nhất là pepton và cao nấm men; Ca2+ và Mg2+ là những yếu tố dinh dưỡng có ảnh hưởng lớn đến quá trình sinh tổng hợp enzym tiêu sợi huyết của chủng. Ở điều kiện nuôi cấy 370C và pH = 6,5, chủng cho hoạt độ enzym tốt nhất ở 24 giờ, chủng nuôi cấy trong bình tam giác 250ml chứa 50ml môi trường cho hoạt độ enzym cao nhất đạt 450±25. FU/ml.
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DOI: https://doi.org/10.54607/hcmue.js.20.3.3756(2023)